FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65271067
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG232050.026.667682
GGCGCGT245800.025.1387793
GCGACAT194850.023.2477727
TGCGACA194050.022.313416
GCGCGTG340500.018.2296054
GTGGCGC364150.017.3926771
CGCGTGC356300.017.3420035
GCTGCGA279400.016.1139014
GGCGATC97250.015.5970911
CTGCGAC286100.015.5214215
GCGTGCC415950.015.3177916
CGTGCCT472350.013.6813077
GCGATCT114050.012.7839632
CGACATC365650.012.7788848
CTCGCGT48300.011.321221
CGATCTG141900.010.4094253
TATCCTA247650.010.3943835
ATCCTAG270900.010.3401726
GGTCGAC69050.010.3292821
GGCTGCG441050.010.1548283
Produced by FastQC (version 0.11.5)