FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62794091
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3324140.5293714658597415No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2385250.37985262020912125No Hit
GCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCT2096860.3339263243734192No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1812190.28859244096709674No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1342670.2138210743428072No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1233230.19639268287202374No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA987220.15721542971296457No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA935160.14892484071470993No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT896670.14279528307846673No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC870410.13861336092913584TruSeq Adapter, Index 2 (100% over 50bp)
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC772690.12305138711220455No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA767130.12216595348119617No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA748910.11926440658246013No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGG684680.10903573713647674No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG682160.1086344254907679No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA672560.10710561922140094No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC638210.10163535928882225No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC631100.10050308714557234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT178050.046.7622036
AGACCGC190450.043.717564
GACCGCG203650.040.8372735
CCGCGTT206350.040.2799157
CAGACCG211050.039.8104823
CGGAGGA603650.037.200372
GAGTAGT620850.036.9347889
GAGAGTA637950.035.8479777
GCGTTCT247150.033.8418249
GGAGAGT704350.032.7517556
AGAGTAG721500.031.9666658
CGCGTTC261150.031.9548458
TCGTATG201550.031.24497842-43
GCGGAGG811750.028.2328741
TATGCCG193100.024.6773846-47
TATCTCG238500.024.16968238-39
ATGCCGT259950.024.06087546-47
AGGAGAG976500.023.8767835
TCTCGTA244050.023.75966840-41
CCGCGCT131000.023.4578382
Produced by FastQC (version 0.11.5)