FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62794091
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1437460.22891644374627543No Hit
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG1207280.19226012842514115No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA1119220.17823651591676037No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1015540.1617254082075971No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT989820.15762948141091812No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT975570.15536015960482652No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG873550.13911340797974128No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC807650.12861878994314926No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA719970.11465569268293094No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG718790.11446777691232125No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG687400.10946889891279739No Hit
GGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCA680040.10829681410628271No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT677260.10785409729077852No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC656960.10462130903367962No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC649650.10345718675981791No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG649350.10340941156390018Illumina Single End PCR Primer 1 (100% over 50bp)
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG638310.10165128435412815No Hit
GGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCT633740.10092350886964827No Hit
CTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAG629280.10021325095700484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT181900.053.869221
GGCGATC244600.039.672322
GGTGGCG589450.031.5784871
TGGCGCG830550.029.6298542
GCGATCT410500.029.126091
GGCGCGT861250.028.6454373
GCCGTAT152250.028.59213646-47
CGTATCA150800.027.85974348-49
CGATCTG435850.027.369362
CGCCGTA134600.027.06585546-47
GTGGCGC926600.026.6990991
GCGCGTG1017600.024.2675464
CGCGTGC1021850.024.157315
GCGTGCC1088950.022.7735486
CCGTATC172100.021.61033648-49
CGTGCCT1158700.021.4352447
TCGCCGT195350.020.93287344-45
GGTCGAC193100.020.5489161
GGTCGCC231150.020.53660642-43
CGGTGGC437450.020.5313781
Produced by FastQC (version 0.11.5)