FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60747357
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1611030.26520166136610684No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1434110.23607776055178828No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1326870.21842431762092956No Hit
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG1294840.21315166024424734No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1227340.2020400657101839No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC1083200.1783122844340372No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG1021650.16818015638112452No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG965570.1589484790260093No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG931630.1533614046780669No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG871560.14347290862382706No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG817770.13461820240179337No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA774250.12745410471109056No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC732630.1206027778294947No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC700200.11526427396668468No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG662870.10911915064880931Illumina Single End PCR Primer 1 (100% over 50bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT626490.10313041273548741No Hit
GCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTT623230.10259376387354596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT169150.038.4582631
GCGATCT357050.036.8246271
GGTGGCG809200.035.9732251
CGATCTG375000.034.6295742
GGCGATC195150.032.882362
CGTATCA149200.028.30262248-49
GCCGTAT154000.027.89784846-47
TGGCGCG1066300.027.847732
GGCGCGT1121650.026.4527363
CGCCGTA136750.026.17211246-47
GTGGCGC1185400.025.2862831
AGGTCGA219900.023.8426076
GCGGTGG492500.023.5998061
GCGCGTG1328950.022.3335844
CGCGTGC1334650.022.2702585
CGGTGGC520600.021.6301062
CCGTATC169350.021.42899948-49
TCGCCGT197150.021.42785644-45
GCTGCGA650200.019.98929
GCGTGCC1496300.019.9024166
Produced by FastQC (version 0.11.5)