FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62656303
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2089990.3335642066210003No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1683530.26869284004835076No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1611150.2571409296204406No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1483050.2366960591339071No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1366000.21801477817802303No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1359000.21689757214050756No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG1341260.2140662528397183No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1340240.21390345995996604No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAA1231360.1965261180507251No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1141670.1822115166929016No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1121080.17892533493398102No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1114560.17788473731046658No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATA1095810.1748922211385501No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT1083440.17291795846939773No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT983290.15693393208980108No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT975610.1557081974657841No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC958880.15303807503612207No Hit
ATTTTTTATTAATTGAAGCACAGAGAAAAGAGGCAAAATGATTAAAGAAA948500.1513814180833491No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT914040.14588157236152283No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT900180.14366950440724216No Hit
GCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCT887360.14162342134996378No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT869140.13871549363517346No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG860190.13728706591577866No Hit
GTCGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTT804500.1283988938830304No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT803820.1282903652965289No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG787990.12576388364311888No Hit
GCACACTTGAGTCTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAG755190.12052897535304628No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT732390.11689007568799582TruSeq Adapter, Index 14 (97% over 44bp)
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG717160.11445935455208713No Hit
GCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATAG684570.10925796244314No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG642930.10261218252854784No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT639670.10209188371679064No Hit
GCACATCACATCAACCTCTGGTCTCACCAATCGGGGGAGGTTTGGGTTGT628590.1003235061602661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC91200.037.6534964
ACCGCGT96450.035.653176
TATGCCG119500.033.01653348-49
TCGTATG125800.032.51757444-45
GTCGGTT65400.031.9893361
GACCGCG115900.029.8338835
GAGAGTA316950.029.3865537
TTAATTG357650.028.1539579
CCGTATC143350.025.94777138-39
GTATGCC158450.025.93625846-47
AGAGTAG368300.025.4441058
GAGTAGT371700.024.9813569
GGGGTCT732150.024.8275871
TATCTCG156900.024.73965840-41
TCCGTAT157050.024.51316836-37
CAGACCG147800.023.9410763
TATTAAT433550.023.6743147
ATTAATT432250.023.6026698
CCGCGTT147300.023.3452017
GCGTTCT151000.023.0877159
Produced by FastQC (version 0.11.5)