FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62656303
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1647170.26288975268777026No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1501510.23964229105569793No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG1474200.2352835915007625No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1349210.2153350796966109No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1267180.20224302094555435No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC1216940.19422467361344317No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1132130.18068892446463047No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG898280.14336626276848796No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG802920.12814672452027692No Hit
GTTTCGTCGAGATGCACACAAGAGTGAGGTTGCTCATCGGTTTAAAGATT802560.12808926820977612No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC800730.12779719863139707No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG777210.12404338634534502No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG761250.12149615657980971No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG731250.1167081307047433No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG702320.11209087775255427No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA670180.10696130603173315No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC647040.10326814207343195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT100100.036.8897971
GGTGGCG764100.036.5220531
GGCGATC106950.034.55752
CGCCGTA89450.033.21126646-47
TGGCGCG1062500.030.4202382
CGGTGGC530800.030.3431021
AGGTCGA201750.030.1791136
GCGGTGG426450.029.8220371
GCCGTAT102700.029.11144646-47
GTGGCGC1151800.028.164921
CGTATCA106100.027.68660748-49
GGCGCGT1180950.027.3575763
GCGATCT177150.026.8005471
GCGCGTG1335450.024.1996654
CCGTATC125250.024.06020448-49
CGCGTGC1375700.023.5331295
GTCTAAC83400.022.9075871
GCGTGCC1439450.022.5205676
GGTCGAC274300.022.0756427
CGATCTG215600.021.9079912
Produced by FastQC (version 0.11.5)