FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72340060
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA3430700.47424621986766397No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3104350.42913290367743684No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC2294140.3171327201000386No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1980400.2737625597766991No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1878390.2596611061699423No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1873700.25901277936457334No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1783500.24654389283061143No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1666490.23036889933461488No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1643530.22719500094415182No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1508290.20849996530276585No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC1057970.14624953310793493No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC982700.13584450994372965No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA795190.10992387896830609No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC740410.10235131129280235No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTCGT728330.10068142050200124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT148250.048.6395156
AGACCGC151200.047.5826574
CCGCGTT154350.046.5598037
GACCGCG158750.045.5876585
CGCGTTC170700.042.350648
GTCGGTT89500.031.3795191
GCGTTCT234550.030.8821959
CAGACCG235100.030.6423283
GAGTCTT1207900.027.031094
GGAGTCT1200950.027.0095023
TGGAGTC1236100.026.1572422
AGTCTTG1297500.025.4749495
GCGTACG33400.023.333432
GTCTTGG1436450.022.9333826
CTCGCTA536900.022.5783543
TCGCTAT557050.022.264914
CGTACGG36300.022.2544823
GTCTCGC566800.022.1545161
TTGGAAG1486650.021.9205979
TCTTGGA1565100.021.2682047
Produced by FastQC (version 0.11.5)