FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63048521
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3406120.5402378907508393No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2751300.43637819830856933No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1579460.25051499622013335No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1457240.23112992610881386No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1285630.2039112067355236No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT964110.1529155616513193No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA943050.14957527711078267No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA921810.1462064431297286No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG808740.12827263624471064No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC773140.122626191342379No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC755480.11982517401161559No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA698060.1107179024865627No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA681310.10806121843841507No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA679070.10770593651197623No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC663990.105314127828629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT381000.056.2120066
AGACCGC391100.054.794994
CCGCGTT392650.054.5145957
CAGACCG416400.051.7965663
GACCGCG436850.049.143475
GCGTTCT466800.046.109449
CGCGTTC493650.043.572658
AATACGG436350.029.59720232-33
TCAGACC769400.028.5824282
CTCAGAC820150.027.8376461
TACGGAG325950.026.81950634-35
AGAACGA486550.026.32160444-45
CAACGCC491750.026.02579982-83
CAATACG436450.025.74356330-31
TACGGGG163050.025.73662834-35
AGCAACG520600.024.57111280-81
CGATCAT527900.024.43967248-49
TGACGGC529100.024.36459562-63
GCGTACG21300.024.3114572
AAGCCGG532250.023.78570692-93
Produced by FastQC (version 0.11.5)