FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63048521
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1057170.16767562239881884No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT982210.15578636650334748No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT951020.1508393828936923No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA887200.1407170201502427No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC814470.12918146010118145No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT795850.12622817908765854No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG793410.12584117556064478No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA775780.12304491646996762No Hit
GGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCA738380.11711297716246191No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA697020.11055295016357324No Hit
GGACGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACG677480.1074537497874058No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC660910.10482561517977558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT190100.054.252321
GGCGATC237300.042.9183732
GGTGGCG564700.029.714931
GCGATCT366900.028.0689873
TGGCGCG698300.027.1472432
GGCGCGT717050.026.4701923
CGATCTG403750.025.649134
CGGTGGC410600.024.3998091
GTGGCGC823500.023.2230531
CGCGTGC868600.022.107775
GCGCGTG874550.021.7462774
GGTCGAC193150.021.6881281
GCGTGCC917450.020.904716
CGTGCCT996600.019.405897
AGGTCGA157750.018.5443576
GCTCATC572900.018.1915456
GCCACGA391300.018.16972218-19
GTCGACC232400.018.1471752
GTAGTCC1026800.017.53244414-15
GGGAAGC635650.017.4890941
Produced by FastQC (version 0.11.5)