FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66471656
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2571060.386790423876306No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1828920.275142836820554No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1623280.24420634262519353No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1530170.23019886852224652No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1505530.22649202541305727TruSeq Adapter, Index 12 (100% over 50bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1312190.1974059439710664No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1157540.1741403884988212No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC980920.14756966488092307No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT970000.14592685941207784No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG960000.14442245879958218No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT921320.13860343723044902No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG886460.13335909669528917No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT854900.12861120836225293No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT854880.12860819956102795No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA817260.12294864445681931No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC797060.1199097552195781No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG711270.10700350236497794No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC698450.10507486077975853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT139450.039.5088736
CCGCGTT150000.036.730087
AGACCGC157400.034.94294
TCGTATG288850.031.83508542-43
GACCGCG176300.031.3046595
CAGACCG181950.030.880753
TATGCCG276950.029.27434346-47
CGCGTTC206350.026.9530288
ATGCCGT339650.026.81468846-47
GTATGCC353500.026.2682744-45
GCCGTCT357800.025.46638748-49
GCGTTCT219600.025.2618779
TCTCGTA337150.025.15347340-41
GTCGGTT74800.025.0033341
AATCTCG347600.024.32514838-39
CGTACGG24550.023.6027983
GAGTAGT301250.023.0029419
CGTATGC380200.021.36201344-45
ATCTCGT363350.021.02537740-41
CGTCTTC445150.020.76768150-51
Produced by FastQC (version 0.11.5)