FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58081440
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC3163440.5446559176218772No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2914820.5018505050838961No Hit
GCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCT2098920.3613753378015421No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1502980.25877113239616645No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA1105210.1902862601202725No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1098750.189174028743089No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1020700.175736001035787No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC965150.16617184422424788TruSeq Adapter, Index 7 (100% over 50bp)
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGA879670.15145457826114506No Hit
GGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCAC875070.1506625868780113No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA857340.1476099766121501No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC829790.14286663691533819No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC795140.1369008757358633TruSeq Adapter, Index 7 (100% over 50bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT725190.12485744155103592No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA708920.1220562024632998No Hit
GCAGCTACTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGA662770.11411046282599055No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGG657480.11319967273538671No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA656100.11296207532044661No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA643670.11082197686558734No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG624180.10746634380965761No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG623400.1073320496186045No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA588820.10137834048191642No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT588460.10131635854758421No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC586810.10103227468189495No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC584420.10062078350674501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT559150.039.295089
GAGAGTA576600.038.0234957
TCGTATG320600.037.2419442-43
GGAGAGT640100.034.474046
CGGAGGA645200.033.936592
AGAGTAG656950.033.5898258
GTATGCC386550.031.29363844-45
ATGCCGT378450.030.55644846-47
ACCGCGT190850.030.4614186
CCGCGTT201900.028.6766367
TCTCGTA342950.028.57358740-41
AGACCGC203650.028.5468274
GCCGTCT400100.028.1529648-49
GCGGAGG827400.027.0146561
CGTACGG38150.026.8918113
AGGAGAG884900.025.2967155
CGCGTTC234200.024.9244658
CAGACCG237100.024.8599973
GCGTACG41750.024.5730382
GACCGCG237350.024.5136345
Produced by FastQC (version 0.11.5)