FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61386380
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGC1326320.21606095684417295No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1241430.2022321563838754No Hit
CAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCT1083670.17653264453776227No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1052350.17143053556831336No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG920920.15002024879134426No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT918900.14969118556917677No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG891170.14517389688070872No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA803060.1308205500959659No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT764010.1244592041426779No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG717680.11691192736890496No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC693340.11294687844437153No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT678350.11050496869175214No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC630740.10274917660888294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC613550.036.1448481
GGCGATC140000.035.1564182
GGGCGAT142800.034.540431
GGTCGAC259100.027.56451
TGGCGCG799850.027.1075674
GGTGGCG832200.026.3404272
GGCGCGT831450.026.0998345
GTCGACC306700.023.5607092
TCGACCC312050.023.4302773
GTGGCGC969700.022.3942663
ACCCGTG330850.021.8950236
GCGCGTG1022550.021.271246
CGCGTGC1071700.020.3607967
GCGTGCC1092950.019.960198
GCACTCG674300.019.5296084
CCGTGCG380250.019.2737068
CGACCCG409900.019.204284
AGGTCGA182100.019.1727396
CACTCGC711500.018.5016045
ACTCGCC720650.018.3770226
Produced by FastQC (version 0.11.5)