FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64602028
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2529780.3915945177448609No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2339580.36215271755865003No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1502250.2325391394833611No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA1450340.22450378802349671No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1445590.22376851698835215No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1413550.21880892036392416No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1397720.21635853289311602No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1286960.19921356029256543No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1281320.19834052268452007No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1256320.19447067513112745No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1126550.17438307045097717No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC711000.11005846441848545No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA683320.10577376920736915No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA670050.10371965412602835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT323250.065.414716
GACCGCG325900.064.888915
AGACCGC331150.063.917594
CCGCGTT336850.062.7459957
CGCGTTC339000.062.4740038
CAGACCG365950.057.6446043
GCGTTCT411650.051.5403149
TCAGACC646700.033.5044332
CTCAGAC753350.030.9671821
AATACGG358500.030.20172132-33
TACGGGG247950.029.97029534-35
CAATACG360750.029.80317130-31
ATACGGG257600.028.46046632-33
GAACGAT383500.027.95894444-45
GCAACGC386550.027.75057280-81
CGCCAAA402650.026.66451384-85
AGAACGA411250.026.44770444-45
AAGAACG408300.026.42357442-43
CAACGCC414750.026.12135782-83
ACGCCAA419050.025.92699284-85
Produced by FastQC (version 0.11.5)