FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58664595
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3280180.5591413355875039No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2881420.4911684807506128No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1951760.332698112038445No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1656460.2823611072402358No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1232000.2100074158868735No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG922990.15733339674466346No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA863480.14718928853084215No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC860030.14660119958213297No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT829860.1414584043408124No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG792870.13515306804726088No Hit
GCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCT791070.13484623903054305No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA721510.12298900214004717No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT718010.1223923901630958No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG672020.11455290878595514No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT662460.11292330578605374No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA641780.10939818130509553No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC635810.10838053173298137No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA633790.10803620139199803No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC619400.10558327386390377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT160250.043.9257776
AGACCGC170000.041.361314
CCGCGTT171000.041.1323747
GACCGCG178950.039.345735
CAGACCG189450.037.892333
CGCGTTC191400.036.797998
GCGTTCT234600.029.9611579
GTCTGGA1208400.025.166341
GTCGGTT73700.024.4820351
CCGCGCT134350.024.2236672
AATACGG187050.023.2347332-33
GAGTAGT357150.022.8453259
CGCGCTG149950.022.5904313
TACGGAG197050.022.308334-35
GAGAGTA367850.021.9225857
CTCGCTA465200.021.3901376
TGGAGTC1595800.021.1202832
GGAGTCT1602150.021.0896823
AACGATC207150.021.01290546-47
CGGAGGA377400.020.8345012
Produced by FastQC (version 0.11.5)