FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66361212
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG5698500.8587094521420133No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC5122140.7718575121864862No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC4526080.6820369706327847No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG4412170.6648718230161318No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG3272790.4931781535273949No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA2493060.37568029950990045No Hit
TGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCG1968330.29660850678857403No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA1674260.2522949701400873No Hit
GTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAAC1275060.19213934790702736No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG1112340.16761900008697853No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG1054590.1589166273816699No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG1014300.15284530969687532No Hit
GTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGC986880.14871337792926384No Hit
CGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCAC860680.1296962448485721No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT800260.1205915286779271No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA798190.12027959947446408No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC793790.11961656155405963No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC782210.11787156629990422No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA751100.11318358682177172No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG718090.10820929551437367No Hit
GGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGG696670.10498150636549557No Hit
GCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCT687930.10366447195087394No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG678330.10221784376090058No Hit
GATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG668300.1007064186832513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG315150.029.2222822
GGCGCGT340100.027.232053
GCGACAT102900.025.3396997
TGCGCTA2310200.024.6195182
GTGCGCT2333400.024.3924561
TTACAGC439950.024.2579082
GCGCTAT2373350.023.960373
GTTACAG462100.023.908531
TACAGCC440750.023.5995713
TGCGACA111100.023.4271166
CGCTATG2476300.023.0045154
TATGCCG2532200.022.5262787
GCTATGC2594200.022.0395765
GCGCGTG428950.021.6467364
CTATGCC2672050.021.4472226
ATGCCGA2770750.020.9073588
TGCCGAT2767100.020.5298659
GTGGCGC460400.020.2518231
CCCCCCG535050.019.4398659
TTCGCGG286400.019.2892242-43
Produced by FastQC (version 0.11.5)