FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58205877
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1468280.2522563142550021No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA636520.10935665482714058No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC610030.10480556800132054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT62250.027.166056
GACCGCG73200.023.3701445
CCGCGTT76050.022.7966737
AGACCGC75750.022.5840074
GCGTACG28350.021.7911432
CGCGTTC80100.021.4068378
GGAGTCT740950.021.337613
GAGTCTT749200.021.0708964
TGGAGTC772250.020.6023772
AGTCTTG808800.019.8995865
CGCACTA169750.017.72596282-83
CGTACGG40850.017.6820853
CCTACGT458650.017.22718824-25
GTCTTGG941850.016.9664156
TTGGAAG950800.016.3354669
CCCTACG464900.016.29063224-25
TCTTGGA1004450.016.2764747
CGATCGG185650.016.23340670-71
GACTACC492700.016.08965518-19
TACGTTC492800.016.07675226-27
Produced by FastQC (version 0.11.5)