FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58205877
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA775830.13329066410252696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT282900.028.978147
TGCGACA297400.027.1506566
GGCGATC96800.024.7055821
GCTGCGA341600.023.950624
GGCGCGT141150.023.4102671
CTGCGAC360050.022.7231085
TGGCGCG151100.020.910262
CGACATC414650.019.9881388
GCGATCT122600.019.1442682
ATCCTAG314950.018.1424676
TATCCTA307450.018.0186165
GTATCCT305600.017.6770024
TGTATCC333900.016.9472163
GGCTGCG493600.016.5079063
TCCTAGG345450.016.2928037
CCTAGGC326650.016.1834498
CTAGGCT323550.016.1036729
CGATCTG153400.015.7960243
CCTGTAT407600.014.9422991
GCGCGTG223450.014.7989572
Produced by FastQC (version 0.11.5)