FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62906922
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1317640.20945866656772683No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1193820.18977561801545464No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT722590.11486653249383272No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC672100.10684038872542514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTA321300.019.41748282-83
GAGTCTT740200.019.0348644
CTCGCTA182800.018.8793071
GGAGTCT755450.018.7638843
TCGCTAT190550.018.18042
GTCGGTT91850.017.8798621
AGTCTTG833400.017.407465
TGGAGTC842150.017.204932
GCGATCG48600.016.9155083
CGATCGG370100.016.83149770-71
CCTACGT425900.016.29356824-25
ATAGCGC398500.015.88822678-79
CCCTACG423750.015.8662324-25
GTCTTGG911050.015.7670876
CGCTATG233600.015.7314481
ACCGCGT48900.015.6397186
CGGCATA402700.015.6045774-75
GGAGTGC1113100.015.4686328
TACGTTC450450.015.43718326-27
ATCGGCA408500.015.41789472-73
Produced by FastQC (version 0.11.5)