FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71808527
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG277400.034.874122
GGCGCGT312050.030.85143
GTGGCGC417250.023.6709961
GCGCGTG442800.021.9012814
CGCGTGC460900.021.1037585
GGCGATC102200.020.56591
GCGTGCC478150.020.553076
TGCGACA231750.019.563846
CGTGCCT570650.017.5899437
GCGACAT271250.017.3473517
GCTGCGA297250.015.6096844
ACCTAGC163500.015.5348296
GCGATCT140350.014.7534212
CTGCGAC321000.014.5736315
GACCTAG188000.013.9449765
CGATCTG161800.013.0331623
TATACGC42400.012.7663957
TATCCTA249150.012.18081955
CCTGTAG721750.011.9416319
GTATCCT264400.011.6574794
Produced by FastQC (version 0.11.5)