FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60481808
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1653920.27345743367989267No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1599730.2644977147508553No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1533650.2535721154367608No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1526710.2524246629664246No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC1336850.22103340561512316No Hit
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG1190730.1968740749284479No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1107100.18304677664397864No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG975240.16124517970759075No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG959340.15861629004212308No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG954270.1577780214506815No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG926790.15323450648168455No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG919640.15205233282708744No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG902900.1492845584245762No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG743100.12286339059176274Illumina Single End PCR Primer 1 (100% over 50bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC706810.11686323927353494No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC697350.115299132592068No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT693650.11468737839318559No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA676370.11183032094543206No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC660810.1092576465306725No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA643520.10639893569319224No Hit
GGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAA638230.10552429252776306No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG636690.10526967050984984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT166450.041.540121
GCGATCT316150.037.593181
GGCGATC183100.037.211562
GGTGGCG901300.035.948121
GCGGTGG576350.034.5557171
CGATCTG362200.032.634182
CGGTGGC607900.032.1774862
CGCCGTA138150.030.92387246-47
GCCGTAT156000.029.68408246-47
CGTATCA156650.029.10646648-49
TGGCGCG1238200.026.6993962
CCGTATC168250.025.6035948-49
GGCGCGT1294600.025.5264913
GTGGCGC1326050.025.0033021
AGGTCGA191450.023.6460366
GCGCGTG1501500.022.0502244
CGCGTGC1512800.021.860375
GGTCGCC236450.020.97876242-43
GCTGCGA596400.020.0032379
GCGTGCC1668500.019.833416
Produced by FastQC (version 0.11.5)