FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62365688
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2370750.38013691118103277No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2352050.37713846754965646No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1844840.29581009352450344No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1318570.2114255518194556No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1174170.18827179458037888No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1124360.18028503108953117No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1118740.1793838945543261No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1086730.17425126457355847No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1045260.1676017748733887No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1025240.1643916763974447No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1008080.16164016341806411No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG951830.15262078083705258No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA951100.15250372929422346No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC899550.14423796623553642No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT764490.12258182736635567No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG758720.12165663914426791No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG719890.11543045913323365No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT707750.11348387594152733No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG632260.10137946365636182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT283150.043.848916
AGACCGC286250.043.1847274
GACCGCG293550.042.3859375
CCGCGTT298750.041.430687
CGCGTTC308250.040.2924968
CAGACCG315500.039.602663
GCGTTCT337400.036.7691849
AATACGG307900.026.90484232-33
TACGGAG316250.026.32110234-35
CTCGCTA573550.023.7093626
GGGGTCT685850.023.6115681
AGAACGA357850.023.23405544-45
TCGCTAT585900.023.1845177
AACGATC360650.022.90205246-47
GTCTCGC605100.022.517514
CAACGCC369950.022.45002282-83
GCGAAGC374850.022.16621876-77
GGTCTCG633900.022.1389883
TCAGACC579800.022.0168422
CGCTATG624800.021.7182468
Produced by FastQC (version 0.11.5)