FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71121442
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA4265620.5997656796666186No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2852350.4010534544561118No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2424730.34092812685097135No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC2381570.3348596334703113No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC2291490.3221939735136416No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG2261550.3179842725910985No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG2144140.30147588964801925No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2027760.2851123294153682No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1927510.27101672094893686No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1771980.24914849167428296No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC1341500.18862103498970115No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1237510.17399956541938505No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC886750.12468110531279723No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC855210.12024643707308409No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC838980.11796442484954116No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA778420.1094494118946576No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA768610.10807008103125917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT295300.063.502176
AGACCGC309900.060.544264
CCGCGTT314400.059.6853687
CGCGTTC315750.059.3549358
GACCGCG336150.055.8580065
CAGACCG369400.050.9474833
GCGTTCT371600.050.306289
TCAGACC594800.032.50462
CTCAGAC665900.031.4984461
GTCGGTT87350.030.0427361
AATACGG330550.029.234132-33
ATACGGA328700.029.06628632-33
TACGGAG338500.028.54695934-35
CAATACG338700.028.2363230-31
GCAACGC340100.028.18336780-81
GAACGAT344100.027.82027444-45
GGAGTCT1112650.026.5855373
CGCCAAA348550.026.36259384-85
GAGTCTT1119950.026.1372554
CAACGCC369900.026.1249982-83
Produced by FastQC (version 0.11.5)