FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71121442
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1712850.24083454325912007No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1696370.23851737989226932No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA1632910.2295946136750152No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1598590.22476906472171923No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1562720.21972557867991482No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG1370170.19265216810424063No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1342040.188696961459246No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC1279960.17996822955305097No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1261910.1774303170062272No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG1227190.17254852622363873No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT1220300.1715797607140755No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG1159160.16298319710671783No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG1074010.1510107176960782No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT977960.13750564843721813No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG890650.12522946314839906No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC876980.12330739863232808No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG811780.11413998045765159No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA767310.10788729508605858No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT759340.10676667663740563No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG735590.10342731802316382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG653150.042.9247742
GTGGCGC704400.039.9139751
GGCGATC146800.035.7990761
GGCGCGT825050.034.0221253
TGCGACA525900.030.3625856
GCGCGTG989450.028.4028324
GCGACAT573250.028.1290577
GCTGCGA571800.027.9674534
GCGATCT194550.026.7319762
CTGCGAC605600.026.4617925
CGCGTGC1191900.023.5867775
CGACATC716950.022.5966648
GCGTGCC1252800.022.5493186
GGCTGCG728600.021.9747433
CGATCTG244300.021.4440253
CGTGCCT1395200.020.3574877
GGTGGCG412100.018.3446871
GGGCGAT135750.018.061451
GACATCT925400.017.7989259
TATCCTA325750.017.1322155
Produced by FastQC (version 0.11.5)