FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66626705
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG2183000.3276463994429861No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1417990.21282607326896927No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1358460.20389121749304576No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1227760.18427445871741668No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1196780.1796246715187251No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1097400.16470873053079843No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC1057130.1586646075323701No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC1033510.15511948249579505No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA959800.1440563509781851No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG849760.1275404509348016No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG795890.11945510437594055No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG778060.11677900025222618No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT772690.11597301712579061No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG762570.11445410665288039No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT692920.1040003404040467No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG679710.10201765193100873No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC679250.10194861054587645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT125500.039.6694261
GGTGGCG607150.037.1458321
TGGCGCG841100.033.1723942
GGCGATC152400.032.4164122
GGCGCGT922950.030.214683
GTGGCGC931000.030.123681
CGGTGGC420300.029.7768961
GCGCGTG1041350.026.8113774
CGCGTGC1073050.026.0622795
GCGTGCC1119550.025.0265336
GCGATCT205200.024.2835433
CGTGCCT1206200.023.243447
GTTACAG447100.022.8020061
TTACAGC433350.022.4382442
GGTCGAC162800.019.861291
AGGTCGA139250.019.6768786
GTAGTCC1302850.019.48050714-15
CGATCTG269350.018.6155284
GTGCCTG1553050.018.4490098
TACAGCC538350.018.3795783
Produced by FastQC (version 0.11.5)