FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76253748
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA175400.014.385231
CGCCGTA71350.014.11293858-59
ATCGGAA198300.012.9382792
GCCGTAT82500.012.40705958-59
CGTATCA85700.012.248631560-61
CGAGTAA94100.011.93798932-33
TCGAGTA102700.011.0539432-33
AGAGCGT231550.010.3808698
GTCGAGT110450.010.36418430-31
GAGCGTC238400.010.04258259
CCGTATC105700.09.99841460-61
AAGAGCG266600.09.5685237
GTGTCGA123250.09.28777428-29
GCGTCGT128350.08.901031510-11
TCGGAAG309300.08.6943843
TCGCCGT123750.08.42481356-57
AGCGTCG147300.08.33640710-11
TCGTGTA137900.08.28402614-15
CGTCGTG147800.07.93830612-13
TGTCGAG144950.07.86460330-31
Produced by FastQC (version 0.11.5)