FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77545874
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA157950.030.8510271
ATCGGAA177550.027.6325862
CGCCGTA91650.020.18686358-59
AGAGCGT236900.019.4276088
GAGCGTC248650.018.4140459
GCCGTAT100600.018.13122258-59
CGTATCA102050.017.40813660-61
TCGGAAG285800.017.299623
AAGAGCG273050.017.2556027
AATTCGT132650.016.59658836-37
GCGTCGT129400.016.22372610-11
GAATTCG146850.015.83260734-35
AGCGTCG146600.015.42181810-11
CCGTATC121650.014.97431560-61
ATTCGTG153450.014.904098536-37
CGTCGTG151550.013.88391912-13
CGGAAGA367700.013.3948614
GTCGTGT160900.013.06235712-13
TCGCCGT146650.012.85885956-57
GTCGCCG183250.012.09209756-57
Produced by FastQC (version 0.11.5)