FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76861066
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG57100.035.4406623
CGTACGT51050.033.9640274
TGCGTAC95100.029.8719562
TTGCGTA71550.026.6300051
ATGCGTA62950.016.077571
TCGTACG29550.013.6643743
CGTACGA28650.011.1089314
TACGTGA135800.09.7939856
CGTACGG39050.09.4884594
TTCGTAC62200.09.39415552
GTACGTG179400.08.8437745
GCGTACC79800.07.79824163
TACGCGA21950.07.3577596
GTGCGTA71200.07.0072211
CGTACGC39200.06.90734054
TCCGATC84750.06.613196434-35
TGCGTAA66350.06.51543572
GCGTACT69200.06.10959243
ACGTGAC188700.06.0664367
TACGTTA52750.05.85318236
Produced by FastQC (version 0.11.5)