FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73629684
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCGG141700.023.0975062
GTCGGGT127800.019.6996574
GTTGTCG141400.016.0919251
TGTCGGG231750.014.6964913
TCGGGTG191950.012.8929695
ATTGTCG73150.08.6369271
CGGGTGA258300.08.5685086
TCCGATC81850.07.544213334-35
GATCGGA135100.06.8213181
CGCCGTA57700.06.668047458-59
GCCGTAT64650.06.171634758-59
ATCGGAA152700.06.0972332
CGAGTAA73750.06.086390532-33
CGATCTA48000.05.88807236-37
TTCCGAT104050.05.774810334-35
CGTATCA68200.05.50220360-61
CCGATCT115150.05.30074136-37
TTTGTCG96800.05.29993251
GTCGAGT86900.05.165379530-31
TCGAGTA83550.05.116654432-33
Produced by FastQC (version 0.11.5)