FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005797423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67874080
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC41350.013.89938732-33
ACGTACG45650.012.902287532-33
GATCGGA103450.011.1665321
ATTACTC251400.010.5325241
TACGCAA61100.09.83418134-35
ATCGGAA125800.09.250682
GTACGCA71750.08.34137234-35
CGTATGC80650.07.92124346-47
TCGTATG79250.07.91135244-45
CTCGTAT81450.07.639347644-45
TTACTCG62350.07.5420072
CGCAAAT80750.07.499951436-37
TATGCCG83750.07.429534448-49
ATTAATC242700.06.26790951
TCGGAAG212050.06.04793743
ACGCAAA106400.05.915139736-37
AATCTCG114700.05.85970640-41
TCTCGTA114400.05.418297342-43
TCACGTA121950.05.297068630-31
TCCGATC66100.05.24563936
Produced by FastQC (version 0.11.5)