FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74070419
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA130650.014.8519231
ATCGGAA148850.013.2767322
TCGTATG90900.011.02531844-45
TATGCCG94300.010.77887548-49
CTCGTAT93400.010.70478144-45
CGTATGC93500.010.59170746-47
ATTACTC262550.08.6586031
TCGGAAG245000.08.6090013
TCTCGTA132550.07.578867442-43
CGGAAGA323800.06.7486224
TTACTCG68200.06.54772142
CGTCTGA168750.06.30483516-17
ATGCCGT176400.06.246845748-49
ATCTCGT168250.06.14013542-43
ACACGTC184850.05.832797512-13
ATTAATC257250.05.7680791
GTATGCC216800.05.17040146-47
ACGTCTG221000.05.03988314-15
TTAATCG50150.05.0205542
Produced by FastQC (version 0.11.5)