FastQCFastQC Report
Thu 21 Jul 2022
EGAF00005797706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45082841
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCCTTATCTCGTAT3328730.7383585253644507TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCCTTATCGCGTAT1842720.40874087770999173TruSeq Adapter, Index 3 (97% over 36bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT608560.1349870563836028No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA475170.10539930258609922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)