FastQCFastQC Report
Thu 21 Jul 2022
EGAF00005797730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45347351
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACAATGATCTCGTAT1438850.31729527045582TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACAATGATCGCGTAT649800.14329392691537812TruSeq Adapter, Index 23 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA562100.12395431874289636No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT547040.12063328682639038No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480990.10606793768394542No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)