FastQCFastQC Report
Thu 21 Jul 2022
EGAF00005797768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39443600
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTCTGCATCTCGTAT1530040.38790576925027126TruSeq Adapter, Index 21 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1519060.38512204768327435No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT903270.2290029307669685No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTCTGCATCGCGTAT816140.20691316208459676TruSeq Adapter, Index 21 (97% over 38bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT486690.12338883874697035No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT485110.1229882667910637No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)