FastQCFastQC Report
Sun 24 Jul 2022
EGAF00005797776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35201850
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGCGTCATCTCGTAT790090.22444559021755958TruSeq Adapter, Index 2 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA634900.18035983904255032No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGCGTCATCGCGTAT469450.13335946832339776TruSeq Adapter, Index 2 (97% over 36bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT413180.11737451298724356No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409180.11623820907139823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)