FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346027882
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGGTCTTGATTAATGAAAACATTC3925350.11344028051473609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGA2131150.018.7727131
GAATAAG2785450.018.7459431
GACTACA2142400.018.6963441
GGTGAGA1516150.018.637174
GACTAAC1446350.018.6183931
AAATGAA1852250.018.5911222
TAGAGAA1347600.018.5265965
AGAAACG1499050.018.4906238
AAAGCAA1697400.018.4599361
CCCAAGA2696250.018.459079
AAACGAG1145950.018.44953210
CACAGGA2138900.018.4330358
AACGAAA920000.018.4140610
CAGATAG1967300.018.4068227
CCGGGAT1801300.018.3950611
CCAAGAT2708200.018.38424710
ACAGGAG2145100.018.3724029
TAGATGA1546050.018.36246910
GCACTCT4535400.018.3539471
GAAGAAA1041600.018.3220926
Produced by FastQC (version 0.11.5)