FastQCFastQC Report
Fri 22 Apr 2022
EGAF00005798421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346027882
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1298050.037.1812449
GGTATCA1503850.031.9505048
TGGTATC1589400.028.5341347
ATCAACG1249550.027.72070510-11
AAGCAGT2091900.026.6172731
CAACGCA1453150.025.45176112-13
TCAACGC1381350.023.92135412-13
GTGGTAT1937800.023.9139756
ACGCAGA1667000.020.89042914-15
AGCAGTG2825750.020.0776842
AACGCAG1903400.018.12762514-15
AGAGTAC1478300.017.27063418-19
CAGTGGT2901600.016.570424
ACGACGC269200.015.9055035
CGCAGAG2012400.015.83345116-17
CTACACG276150.015.6501971
AGTGGTA3454700.014.26106555
TATCAAC1934900.013.55352610-11
CAGAGTA1842800.013.17064318-19
GCAGTGG4724650.012.1985893
Produced by FastQC (version 0.11.5)