FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353166938
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1347000.037.9209989
GGTATCA1593500.031.6954258
TGGTATC1610100.030.40177
ATCAACG1320050.027.14737910-11
AAGCAGT2218100.027.0043661
CAACGCA1508900.025.08406312-13
GTGGTAT2031200.024.6879046
TCAACGC1466100.023.16351112-13
ACGCAGA1755350.020.43990114-15
AGCAGTG3070200.019.794422
AACGCAG1959600.018.03838214-15
AGAGTAC1574700.017.16225418-19
CTACACG293250.016.6326981
CAGTGGT3158000.016.3952454
ACGACGC293450.016.0709925
CGCAGAG2145450.015.28818516-17
AGTGGTA3717250.014.32016855
TATCAAC1994800.013.86372910-11
CAGAGTA1934850.013.26718418-19
GCAGTGG5068750.011.9384063
Produced by FastQC (version 0.11.5)