FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355235936
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTTAAGTTTCAGCTTTGCAACC3637180.10238772689934161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAG1707000.018.7860741
GATGAAA1656850.018.6819691
ATGAAAT1274400.018.2807032
AGAAACC1973650.018.2667168
TATATGA1550900.018.2660629
GGAAAGA866500.018.13758510
CTGCCTA2165950.018.1311381
GACTAGA1342700.018.0563149
GCCGAAG906550.018.0124953
AGCTACA1328600.017.9819663
AGACTAG1382200.017.9780398
CACGAGA1099850.017.9094248
CGTGCGA1168850.017.8844077
CTCGGGA1518400.017.8782221
AAGACTA1059400.017.8692537
GCGAGAA793550.017.8684841
CTCGAAA980200.017.8651961
CGGACGT1511450.017.8593921
CGTTGGG2314550.017.8556581
CGCCGAA898950.017.8527282
Produced by FastQC (version 0.11.5)