FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355235936
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1371750.037.7504929
GGTATCA1581050.032.6078348
TGGTATC1578700.030.7747827
ATCAACG1305650.027.9383210-11
GTGGTAT1928300.025.726526
CAACGCA1522700.025.59326212-13
AAGCAGT2310450.025.548121
TCAACGC1477550.023.30597512-13
ACGCAGA1693850.021.56265314-15
AGCAGTG2981200.020.1255512
AACGCAG1942200.018.5142814-15
CAGTGGT2941350.017.6393074
AGAGTAC1566600.017.52075218-19
ACGACGC268000.017.424165
CTACACG278200.017.0570451
CGCAGAG2060900.016.14718816-17
AGTGGTA3406250.015.3784275
CAGAGTA1893550.013.66134218-19
TATCAAC2073100.013.46236410-11
GTACTTT1731900.012.729526522-23
Produced by FastQC (version 0.11.5)