FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349326927
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCC5025270.1438557869889028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGA1452050.018.1156731
CAATGGA1510250.017.9789318
GATGAAA871250.017.9030231
CGGAGCT1597050.017.6219391
GAATGAA738900.017.5963691
TATTGAG922850.017.4786013
CAATGAA702750.017.3953238
CGCTGGA1062800.017.343661
ATAACGC1349800.017.2364651
CTTAACT1480400.017.1868821
TGACTAG1044300.017.1614721
ACTGAAC755250.017.1226751
AAATGAG594600.017.1130162
GGAACTT1449850.017.0950931
TGCCAAA1267900.017.085231
TCTGGAA1025200.016.9836061
GAACCTA1157800.016.8529191
TGGCTGG1566100.016.85061
CGCTTCA1338550.016.8498421
TGGGCGT1465650.016.832711
Produced by FastQC (version 0.11.5)