FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349326927
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7406300.2120162926919172No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5314960.15214859174025255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1804750.060.4530759
GGTATCA2110150.050.6764838
TGGTATC2066050.049.481227
GTGGTAT2548100.040.58616
AAGCAGT3010350.040.253741
ATCAACG1881900.036.6535810-11
AGCAGTG3491150.034.286272
CAACGCA2179050.033.6175712-13
TCAACGC2085500.031.9975612-13
ACGCAGA2393550.029.1649914-15
AACGCAG2440050.028.47697314-15
CAGTGGT3864200.027.7931334
GCAGTGG4563250.025.8299223
AGAGTAC2159450.025.30475418-19
TATCAAC2330350.024.93763710-11
CGCAGAG2702500.024.13821816-17
GTACTTT1978600.023.761922-23
GCAGAGT2403500.023.19984616-17
CAGAGTA2362250.022.67419818-19
CTACACG247750.021.0614151
Produced by FastQC (version 0.11.5)