FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344132925
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCC3876130.11263467452293326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAAG6350000.019.3033621
AAACCTG8442850.019.2754081
AGGCCAC8907800.019.248151
GGGAATG4380300.019.2469041
GTTAAGC6350900.019.193561
ACGGAGA3221800.019.1931231
GTCCCAG5971050.019.0876372
AGGCGAA2401550.019.05503810
TGTCCCA6373000.019.0522041
AGACTAG4595800.019.029678
GACTAGA4551250.019.0012519
CTGGTTA8129800.018.9922315
ATAGACC2991400.018.9716781
ACCTTGA4591600.018.9689252
TTGAGAC4489100.018.9644055
AGGGCTT8592350.018.9629338
ACCATTA5287850.018.9524756
CCTTGAG4514400.018.9521623
CCATTAA5249000.018.9403387
ATGCGAG2485800.018.924643
Produced by FastQC (version 0.11.5)