FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344132925
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9428420.2739761096675071No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC4312040.12530158223018037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2265200.055.4069189
GGTATCA2358150.052.6236768
TGGTATC2364200.050.8623777
GTGGTAT2671050.045.532386
AAGCAGT3086650.043.6575281
AGCAGTG3775750.035.5735742
ATCAACG2164300.035.23867410-11
CAGTGGT3762150.032.9649854
TCAACGC2299450.032.3409312-13
CAACGCA2489900.032.28581612-13
AGTGGTA4053000.030.5909585
ACGCAGA2638250.029.38052714-15
AACGCAG2728300.028.00061614-15
AGAGTAC2375400.027.1001818-19
CGCAGAG3043200.024.22751216-17
TATCAAC2808050.023.50336510-11
GCAGTGG5974900.022.4798373
GTACTTT2410900.022.38701222-23
CAGAGTA2811250.022.28958518-19
GCAGAGT3105600.021.22175616-17
Produced by FastQC (version 0.11.5)