FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351141073
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT1596850.018.0506341
CTGCCTA1597150.017.9645291
TGCCAAA1564450.017.8409211
TGACTAG1601200.017.6752681
TGCTACC1707250.017.456231
CGCTGGA1247950.017.342061
CCTTACG1551250.017.3324131
CGGAGCT1239100.017.2590561
TGGCTGG1737000.017.073251
ACTGAAC965100.017.0381131
TTGCCGT1553350.016.9186521
GATCTAG1564550.016.8655911
GATGAAA1018850.016.8611161
CTAGCCT1585350.016.856371
AAGAATC1277800.016.7983610
GACCTGG1203800.016.7078631
CAAAGAA1289150.016.6993398
CATGGCG1260050.016.6677421
ATGCGAT925150.016.662391
GACCAAT1456700.016.6469941
Produced by FastQC (version 0.11.5)