FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351141073
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1423900.031.5923149
ATCAACG1151500.027.91529810-11
GTGGTAT1639500.026.0660346
AAGCAGT2151850.025.001061
CAACGCA1389250.024.72316412-13
TGGTATC1730750.024.5356717
GGTATCA1858550.024.0756918
TCAACGC1308350.023.31090712-13
ACGCAGA1623200.019.99075914-15
AACGCAG1616150.019.50718114-15
AGCAGTG2857550.018.8801022
CTACACG257050.018.550771
ACGACGC262500.017.7236255
AGAGTAC1339800.017.6849218-19
CAGTGGT2802800.015.8303974
AGTGGTA2873300.015.62146955
CGCAGAG1942800.015.10764316-17
TATCAAC1632100.014.94773210-11
GCAGTGG4117450.012.9639043
TACACGA384950.012.3007682
Produced by FastQC (version 0.11.5)