FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360575496
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACC6134100.17011971329299647No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT4585200.12716338328215182No Hit
CCACCTACTAAGAACGGCCATGCACC4356430.1208188035051611No Hit
ACCTTTAAGTTTCAGCTTTGCAACC4236210.11748468897620265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT1486450.017.9917281
GAATGAA1230750.017.905811
GGGAGAT1519250.017.7836361
TCTGGAA1317100.017.6601051
TACTTGT2152300.017.5902631
ACTGAAC1075050.017.5272961
TGCCAAA990150.017.4957661
GATGAAA729150.017.366391
GAACCTA1750250.017.3229371
CAATGAA761400.017.2982568
CTGATAG1182250.017.2875181
AGATAGA786700.017.2699284
TTGGAAC1276900.017.2493571
TGACGGC1271650.017.2124541
CCACCTA1780400.017.1673621
CGACCTT1692200.017.1245841
ACCAGTA1841600.017.052851
TCGCAAA887250.016.9785868
CCTTACA1402850.016.923243
AGAGACT1425400.016.9028768
Produced by FastQC (version 0.11.5)