FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360575496
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT5271690.1462021146328812No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4744780.13158908613135487No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA4698040.13029282500106443No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT4600650.12759186497797953No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG4452150.12347344867827624No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT4211410.11679689958743066No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA4153130.11518059452381646No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC4053140.11240752754868291No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT3844000.10660735525965968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1431600.052.5483479
GGTATCA1862900.040.109528
GTGGTAT1871500.039.0343256
TGGTATC1853600.038.8249557
AAGCAGT2240100.037.5138851
ATCAACG1517950.032.08669310-11
CAACGCA1700750.029.83201412-13
TCAACGC1670050.028.04471812-13
AGCAGTG3065250.027.4642622
ACGCAGA1930900.025.2273814-15
AGAGTAC1603500.023.13159218-19
AACGCAG2146450.022.59374614-15
CTACACG292900.022.554881
ACGACGC293650.022.3570065
CAGTGGT3357750.022.2184644
GCAGTGG4097450.020.305713
AGTGGTA3713250.020.1510965
CGCAGAG2295550.019.79857316-17
CAGAGTA1890100.019.01970318-19
TATCAAC2180300.018.40507910-11
Produced by FastQC (version 0.11.5)