FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371433347
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACC4223220.11370061503928457No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT3870020.10419150653158775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC1024250.017.8914341
CTCGAAA916000.017.8435861
ACTGAAC1119500.017.801741
GATGAAA1037350.017.7095831
GAATGAA781000.017.4282841
CCACCTA1823650.017.3211981
GGGATGA992650.017.29711
ATGCGAT1018800.017.239591
CGACCTT1703600.017.2093771
ACTGATG959150.017.0097451
CGGCCGA931400.016.9877669
GACTGCG1518550.016.9782281
GGCGACT868000.016.9483261
CACCGAA704050.016.9321568
CGTTGGG2102300.016.8939481
TGCGGGT1641550.016.8605021
CAATGGA1122150.016.8382458
AAATGCC1826750.016.8215451
CGATTGA1174250.016.8057691
GGTATTG1538900.016.7453141
Produced by FastQC (version 0.11.5)