FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371433347
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1032400.038.168649
ATCAACG915300.029.40818410-11
CAACGCA996500.028.73865112-13
TCAACGC972300.026.50925312-13
GGTATCA1478650.026.435848
GTGGTAT1528500.024.6213026
AAGCAGT2064750.022.2960661
TGGTATC1777800.021.1944967
AACGCAG1461350.018.7494914-15
AGAGTAC1127050.018.3039618-19
ACGACGC244300.018.19275
ACGCAGA1543650.018.03009414-15
AGCAGTG2670700.017.2870182
AGTGGTA2325050.016.9087565
CGCAGAG1679950.015.49262816-17
CAGTGGT2735150.014.3393324
GTACTTT1479950.012.10497622-23
CACGACG369600.012.1002184
TATCAAC1884450.011.53812410-11
GCAGTGG4097000.011.31244853
Produced by FastQC (version 0.11.5)