FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344498411
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6244480.18126295508515422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3008300.050.5128631
GTATCAA2444100.049.852529
GGTATCA2556100.046.5623178
TGGTATC2619950.043.2744647
GTGGTAT2659050.043.1219376
AGCAGTG3821850.038.760742
ATCAACG2914900.031.9209910-11
CAACGCA3290050.030.31564912-13
CAGTGGT3965250.029.7446064
GCAGTGG4821450.029.0487863
ACGCAGA3362750.029.03282414-15
TCAACGC3031600.028.45956212-13
AACGCAG3456450.026.38002614-15
AGAGTAC2689500.025.87807818-19
CGCAGAG3533500.024.88426216-17
AGTGGTA5491900.022.0633355
CAGAGTA3036600.021.63372418-19
GCAGAGT3401900.021.20565616-17
TATCAAC3186400.020.88521810-11
CTACACG283350.020.8555491
Produced by FastQC (version 0.11.5)